Flavonoid 3',5'-Hydroxylase (<i>F3'5'H</i>) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [<i>Eustoma grandiflorum</i> (Raf.) Shinn.]

This study investigated genetic polymorphism related to anthocyanin composition variation in lisianthus (Eustoma grandiflorum) flower petals. Three different bands were detected by genomic polymerase chain reaction using specific primers based on the reported flavonoid 3',5'-hydroxylase (F3'5'H) gene sequence. Our revealed that they allelic. They named F3'5'H1-1, F3'5'H1-2, and f3'5'h1-2. F3'5'H1-1 F3'5'H1-2 differed length of intron. Allele f3'5'h1-2 possessed a retrotransposon insertion first exon, but otherwise, its sequence was almost identical F3'5'H1-2. is Ty1-copia type designated as Eustoma grandiflorum 1 (rTeg1). The mauve line ‘ME’ red-purple ‘PRP’ homozygous for mostly accumulated cyanidin, with small amount delphinidin, petals (the Cy major phenotype). violet ‘RV’ only delphinidin Dp pink-red ‘AK’ f3'5'h1-2, pelargonidin, lacked Pg major-non Segregation analysis F2 population from × associated phenotype, trait dominant phenotype. All phenotype plants F3'5'H1-1. Genetic cross between delphinidin-containing delphinidin-lacking strains genotype co-segregated which recessive phenotypes. Expression F3'5'H demonstrated an abnormal mRNA transcribed rTeg1. Therefore, these polymorphisms can be used DNA markers breeding color.